Highly accomplished Assistant Professor and Research Scientist with over 8 years of postdoctoral and project leadership experience in Biotechnology, specializing in drug target discovery, structural biology, gene editing, and RNA editing. Recognized for significant contributions to filarial parasite biochemistry, trypanosome RNA editing, and COVID-19 drug repurposing, evidenced by 20 research articles, 2 review articles, 2 book chapters, and a Google Scholar profile with 418 citations, an h-index of 12, and an i10-index of 16.
Work
Deen Dayal Upadhyay Gorakhpur University
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Assistant Professor
Gorakhpur, Uttar Pradesh, India
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Summary
Leads academic instruction and research initiatives for postgraduate biotechnology students at a prominent state university.
Highlights
Delivered advanced lectures and practical sessions to postgraduate biotechnology students, fostering critical thinking and research skills for approximately 50+ students per year.
Mentored and guided numerous postgraduate students in their research projects, contributing to their academic success and scientific development.
Contributed to departmental curriculum development and academic administrative duties, ensuring high educational standards and smooth operational flow.
Translational Health Science and Technology Institute
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Project Scientist-II
Faridabad, Haryana, India
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Summary
Directed and executed complex research projects focused on translational health, delivering critical experimental data and analysis.
Highlights
Managed and executed complex research projects, ensuring adherence to scientific protocols and delivering critical experimental data within an 11-month tenure.
Applied advanced biochemical and molecular biology techniques to investigate disease mechanisms, contributing to the institute's research pipeline.
Collaborated effectively with interdisciplinary teams, accelerating research progress and facilitating knowledge exchange in a fast-paced environment.
University of Allahabad
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Postdoctoral Researcher
Allahabad, Uttar Pradesh, India
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Summary
Conducted independent biochemical research, advancing understanding of molecular mechanisms and contributing to significant findings.
Highlights
Spearheaded independent research in biochemistry, resulting in 6 peer-reviewed research articles and 1 review article, significantly advancing scientific understanding during a 3-year tenure.
Conducted extensive *in silico* studies for drug repurposing against SARS-CoV-2 and gastric cancer, identifying potential therapeutic compounds.
Utilized advanced techniques in protein analysis, enzyme kinetics, and molecular docking to elucidate structure-function relationships of various biomolecules.
Texas A&M University
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Postdoctoral Researcher
College Station, Texas, US
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Summary
Executed cutting-edge biochemical and biophysical research, contributing to significant advancements in understanding complex biological systems.
Highlights
Conducted pioneering research on RNA editing helicase 2 subcomplex in Trypanosome holo-editosomes, publishing 2 high-impact research articles and 1 review article in leading journals.
Applied sophisticated biophysical and molecular biology methodologies to unravel site-specific and substrate-specific control of mRNA editing, contributing to fundamental biological insights.
Collaborated with international research teams, presenting findings at conferences and contributing to a book chapter on RNA metabolism in mitochondria.
Education
Banaras Hindu University
Varanasi, Uttar Pradesh, India
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Ph.D.BiochemistryGrade:
Banaras Hindu University
Varanasi, Uttar Pradesh, India
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Master of Science (M.S.)BiochemistryGrade:
Awards
ICMR-Junior Research Fellowship
Indian Council of Medical Research
Secured a competitive national fellowship to support biomedical research, demonstrating high potential in the field.
CSIR-Junior Research Fellowship
Council of Scientific and Industrial Research and University Grants Commission (CSIR-UGC)
Awarded a prestigious national fellowship for doctoral research in scientific disciplines, recognizing exceptional academic merit.
NET-LS (National Eligibility Test for Lectureship)
Council of Scientific and Industrial Research and University Grants Commission (CSIR-UGC)
Qualified for eligibility as a lecturer in Indian universities and colleges, demonstrating high academic aptitude and subject mastery.
Publications
Biophysical and in-silico studies on the structure-function relationship of Brugia malayi protein disulfide isomerase.
Journal of Biomolecular Structure & Dynamics
Doharey PK, Verma P, Dubey A, Singh SK, Kumar M, Tripathi T, Alonazi M, Siddiqi NJ, Sharma B. J Biomol Struct Dyn. 2023 Apr 20:1-11. doi: 10.1080/07391102.2023.2201849. [I.F. 5.235]
Biochemical Characterization of Different Chemical Components of Parthenium hysterophorus and Their Therapeutic Potential against HIV-1 RT and Microbial Growth.
BioMed Research International
Jaiswal J, Doharey PK, Singh R, Tiwari P, Singh N, Kumar A, Gupta VK, Siddiqui AJ, Sharma B. Biomed Res Int. 2022 Apr 28;2022:3892352. doi: 10.1155/2022/3892352. PMID: 35528165; PMCID: PMC9071890. [I.F. 2.583]
Pharmacological Chemistry and Biomedical Implications of Chemical Ingredients from Parthenium hysterophorus.
Current Topics in Medicinal Chemistry
Jaiswal J, Singh N, Gupta VK, Doharey PK, Siddiqi NJ, Sharma B. Curr Top Med Chem. 2022 Mar 7. doi: 10.2174/15680266226662203071450 27. Epub ahead of print. PMID: 35255797. [I.F. 3.295]
Repurposing of gastric cancer drugs against COVID-19.
NADP⁺ binding effects tryptophan accessibility, folding and stability of recombinant B. malayi G6PD.
International Journal of Biological Macromolecules
Verma A, Chandra S, Suthar MK, Doharey PK, Siddiqi MI, Saxena JK. Int J Biol Macromol. 2016 Apr; 85:645-54. doi: 10.1016/j.ijbiomac.2015.12.087. Epub 2016 Jan 4. PMID: 26763177. [I.F. 8.025]
Designing, synthesis of selective and high-affinity chalcone-benzothiazole hybrids as Brugia malayi thymidylate kinase inhibitors: In vitro validation and docking studies.
Synthesis, molecular docking and Brugia malayi thymidylate kinase (BmTMK) enzyme inhibition study of novel derivatives of [6]-shogaol.
European Journal of Medicinal Chemistry
Singh VK, Doharey PK, Kumar V, Saxena JK, Siddiqi MI, Rathaur S, Narender T. Eur J Med Chem. 2015 Mar 26;93:74-82. doi: 10.1016/j.ejmech.2015.01.035. Epub 2015 Jan 20. PMID: 25659753. [I.F. 7.1]
Operative conversions of 3-carboxy-4-quinolones into 3-nitro-4-quinolones via ipso-nitration: potential antifilarial agents as inhibitors of Brugia malayi thymidylate kinase.
RSC Advances
CS Azad, VM Balaramnavar, IA Khan, Doharey PK, JK Saxena, A K Saxena. RSC Advances 2015, 5 (100), 82208-82214. doi.org/10.1039/C5RA18036H. [I.F. 4.036]
Identification of novel PTP1B inhibitors by pharmacophore-based virtual screening, scaffold hopping and docking.
Filariasis: Biochemistry and Chemotherapeutic Targets.
Nova Publisher USA
Jitendra Kumar Saxena, Anita, Manish Kumar Suthar, Pawan K. Doharey, Alok R. Singh. In Nematodes: Morphology, Functions and Management Strategies. Publisher: Nova Publisher USA. ISBN 978-1-61470-784-4. 83-107, 2013.